Injection of An. stephensi Embryos to Generate Malaria-resistant Mosquitoes
نویسندگان
چکیده
منابع مشابه
Injection of An. stephensi embryos to generate malaria-resistant mosquitoes.
The introduction of exogenous genes into the genomes of mosquitoes requires microinjection techniques tailored to the specific species of interest. This video protocol demonstrates a method used by the James laboratory to microinject DNA constructs into Anopheles stephensi embryos for the generation of transformed mosquitoes. Techniques for preparing microinjection needles, collecting and prepa...
متن کاملInsecticide-resistant mosquitoes and malaria control.
The emergence of insecticide-resistant mosquitoes strongly challenges the fight against mosquito-borne diseases, in particular malaria. In this paper, we formulate a system of nonlinear difference equations for malaria transmission cycle. Our model incorporates compartments for insecticide-resistant mosquitoes, where mutation is the only evolutionary force involved in the occurrence of resistan...
متن کاملLaser induced mortality of Anopheles stephensi mosquitoes.
Small, flying insects continue to pose great risks to both human health and agricultural production throughout the world, so there remains a compelling need to develop new vector and pest control approaches. Here, we examined the use of short (<25 ms) laser pulses to kill or disable anesthetized female Anopheles stephensi mosquitoes, which were chosen as a representative species. The mortality ...
متن کاملAnnotated Differentially Expressed Salivary Proteins of Susceptible and Insecticide-Resistant Mosquitoes of Anopheles stephensi
Vector control is one of the major global strategies for control of malaria. However, the major obstacle for vector control is the development of multiple resistances to organochlorine, organophosphorus insecticides and pyrethroids that are currently being used in public health for spraying and in bednets. Salivary glands of vectors are the first target organ for human-vector contact during bit...
متن کاملFrom noxiustoxin to scorpine and possible transgenic mosquitoes resistant to malaria.
Scorpion venom contains different types of peptides toxic to a variety of organisms whose molecular targets have been described as mainly ion-channels of excitable cells where they cause impairment of function. Based on mouse, cricket, and crustacean bioassays, specific toxins for each group of animals have been found. Chromatographic techniques were used to isolate and chemically characterize ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Visualized Experiments
سال: 2007
ISSN: 1940-087X
DOI: 10.3791/216